Agilent, GK80030, AdvanceBio Sialidase C, 1 U
AdvanceBio Sialidase C, 1 U
Catalog Number: GK80030
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Category
Pack Size40 units / pack
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| Concentration | 10 U/mL |
|---|---|
| Enzyme Formulation | 20 mM Tris HCl pH 7.5, containing 25 mM NaCl |
| Enzyme Source | Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24. Sialidases are also known as neuraminidases. |
| Enzyme Specific Activity | ≥40 units/mg |
| Enzyme Specificity | This enzyme releases a2-3, and a2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc a2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. Sialidase A (GK80040) is recommended for this application. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5 |
| Molecular Weight | ~41,000 daltons |
| Unit | 1 U |
| Volume | 100 µL |
| pH Optimum | 6 |
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