Agilent, GKE-5006D, AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

Name: AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
Brand: Agilent
SKU: GKE-5006D

AdvanceBio N-Glycanase, PNGaseF, 1U

Catalog Number: GKE-5006D

Pricing

Concentration	2.5 U/mL
Enzyme Applications\|MultiLine	Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies\|Release of intact N-linked glycans from glycopeptides and glycoproteins\|Structure-function studies of N-glycosylated glycoproteins\|
Enzyme Formulation	20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity	≥10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.

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