| Size | 1EA |
|---|---|
| Description | 1/16” connections |
| Column Format | Analytical |
| Phase | C4 |
| Pore size | 300 Å |
| Particle Size | 5 µm |
| Ø int. | 4,60 mm |
| Length | 150 mm |
Answer:An analytical 150 × 4.6 mm column packed with 5 µm silica particles.
Answer:The butyl-bonded phase provides relatively weak hydrophobic retention for peptides and proteins.
Answer:Yes. The stationary phase has a 300 Å pore size.
The ACE C4-300 HPLC column features a butyl-bonded reversed-phase silica medium with 300 Å pores for analytical separations of peptides and proteins. The 150 × 4.6 mm column is packed with 5 µm particles and suits conventional HPLC instrumentation. Its lower hydrophobic strength than C8 or C18 can help elute strongly retained proteins under milder organic-solvent conditions. The combination of wide pores and base-deactivated silica makes the C4-300 phase a practical option for comparing protein retention and selectivity in reversed-phase method development.
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