BD, 347301, Biotin Mouse Anti-Human CD5 L17F12

Name: Biotin Mouse Anti-Human CD5 L17F12
Brand: BD
SKU: 347301

100 Tests

Catalog Number: 347301

Pricing

Verified Reactivity	Human
Reported Reactivity	African Green, Baboon, Cynomolgus, Rhesus
Antibody Type	Monoclonal
Host Species	Rat
Immunogen	C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells.
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography.
Concentration	0.5 mg/ml
Storage & Handling	The antibody solution should be stored undiluted between 2°C and 8°C.
Application	FC - Quality tested
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes	It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151.

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