BD, 347321, Biotin Mouse Anti-Human CD4 SK3 (also known as Leu3a)
100 Tests
Catalog Number: 347321
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151. |
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| Application References(PubMed link indicates BioLegend citation) | Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed |
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| Product Citations | Thompson EN, et al. 2023. Exp Hematol. 36:124. PubMed Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed |
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| RRID | AB_2687395 (BioLegend Cat. No. 347321) AB_2687396 (BioLegend Cat. No. 347322) |
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| Structure | Single-pass type I membrane protein and is 150 kD. |
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| Distribution | Monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts. |
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| Ligand/Receptor | Macrophage Colony Stimulating Factor (M-CSF) and IL-34. |
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| Cell Type | Dendritic cells, Macrophages, Monocytes, Osteoclasts |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Sherr CJ, et al. 1989. Blood 73:1786 2. Roussel MF, et al. 1991. Nature 353:361. 3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924. |
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| Gene ID | 1436 |
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| UniProt | View information about CD115 on UniProt.org |
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| Clone | 9-4D2-1E4 |
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| Regulatory Status | RUO |
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| Workshop | V MA199 |
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| Other Names | M-CSFR,CSF-1R, FMS, CSF-1 Receptor |
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| Isotype | Rat IgG1, κ |
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