BD, 553446, Purified Rat Anti-Mouse IgG2a R11-89

The R11-89 antibody recognizes mouse IgG2a, with strong reactivity to the Igh-1[a] allotype and weaker reactivity to Igh-1[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
For the sandwich IgG2a ELISA, R11-89 mAb is optimal for capture with biotin-, AKP-, or HRP-conjugated anti-mouse IgG2a R19-15 mAb (Cat. No. 553388, 553389, or 553391, respectively) for detection. This pair of anti-mouse IgG2a mAbs can effectively quantitate mouse IgG2a of these mouse Igh-C haplotypes, in order of sensitivity from highest to lowest: e, a, j, and b.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.