BD, 562607, PE Mouse anti-Mouse RORγt Q31-378.262.80

Name: PE Mouse anti-Mouse RORγt Q31-378.262.80
Brand: BD
SKU: 562607

50 µg

Catalog Number: 562607

Pricing

Send an RFQ.

View RFQ List

Category

Fluorescent-Conjugated Antibodies for FACS

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The Q31-378 monoclonal antibody recognizes mouse RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.
Optimal staining occurs when using the PE Mouse anti-Mouse RORγt antibody with cells that have been fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574). However, staining with this fluorescent antibody is also compatible when using target cells that have been fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. Cy is a trademark of GE Healthcare.
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Specifications

General

Brand	BD Pharmingen™
Alternative Name	RORγT; RORgt; RORgamma t; RORgammaT; Rorc2; Rorg; TOR; Thor; Nr1f3
Reactivity	Mouse (QC Testing)
Isotype	Mouse IgG2a, κ
Immunogen	Mouse RORγt Recombinant Protein
Application	Intracellular staining (flow cytometry) (Routinely Tested)
Concentration	0.2 mg/ml
RRID	AB_11153137
Storage Buffer	Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status	RUO

FAQs

Question: What target and species are recognized by the PE RORγt antibody?

Answer:Clone Q31-378 recognizes mouse RORγt, a nuclear transcription factor associated with Th17 and other lymphoid populations.

Question: What are the antibody conjugate and supplied quantity?

Answer:The reagent is PE-conjugated mouse IgG2a κ monoclonal antibody supplied as 50 µg at 0.2 mg/mL.

Question: Is intracellular staining needed to detect RORγt?

Answer:Yes. Nuclear RORγt detection requires an appropriate cell fixation and permeabilization workflow before staining.

Application Note

BD Pharmingen PE Mouse Anti-Mouse RORγt is a phycoerythrin-conjugated monoclonal antibody for detecting intracellular mouse RORγt by flow cytometry. Clone Q31-378 recognizes the RORγt transcription factor associated with Th17 differentiation and selected other lymphoid-cell populations. The reagent is a mouse IgG2a κ antibody supplied as 50 µg at a concentration of 0.2 mg/mL. As RORγt is a nuclear transcription factor, staining requires a compatible fixation and permeabilization procedure before intracellular antibody labeling. PE fluorescence enables RORγt expression analysis in appropriately configured cytometers and multicolor panels. Panel design should include appropriate fluorescence controls, compensation, and a validated antibody titration to distinguish true intracellular signal from nonspecific binding.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.