BD, 554002, HRP Goat Anti-Mouse Ig Poly1270

Name: HRP Goat Anti-Mouse Ig Poly1270
Brand: BD
SKU: 554002

1 ML

Catalog Number: 554002

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Category

Western Blot Detection Reagents

TagsReagents western-blotting-and-molecular-reagents

CollectionsBD Western Blot Reagents: Membranes, Buffers, ECL & Antibodies BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

Store undiluted at -20°C. Avoid multiple freeze-thaws of product. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. ProClin is a trademark of Rohm and Haas Company.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Specifications

General

Source	Mouse LIF, amino acids (Ser24-Phe203) (Accession# NP_032527.1), was expressed in E. coli.
Molecular Mass	This 180 amino acid protein has a predicted molecular mass of approximately 20 kD. The protein migrates at approximately 20 kD in DTT-reducing conditions and approximately 18 kD in non-reducing condition by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity	>98%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in PBS, pH 7.4.
Endotoxin Level	Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration	10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
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