BD, 554006, Purified Mouse Anti-Armenian and Syrian Hamster IgG1 G94-56

Name: Purified Mouse Anti-Armenian and Syrian Hamster IgG1 G94-56
Brand: BD
SKU: 554006

0.5 mg

Catalog Number: 554006

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Substrate Solutions

TagsReagents immunoassay-reagents

CollectionsBD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels BD Cell Preparation & Separation Reagents (PBMC, Magnetic, RBC Lysis)

Description

Based on ELISA, the G94-56 antibody reacts specifically with Armenian and Syrian hamster IgG1 monoclonal antibodies, but has low reactivity with some other hamster IgG groups. The G94-56 mAb does not react with hamster IgM.
For the hamster IgG (group 1) sandwich ELISA, biotin-conjugated mAb G94-56 (Cat. No. 554007) is optimal for detection with purified anti-hamster IgG mAb HIG-632 (Cat. No. 550637) for capture.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Specifications

General

Source	Mouse LIF, amino acids (Ser24-Phe203) (Accession# NP_032527.1), was expressed in E. coli.
Molecular Mass	This 180 amino acid protein has a predicted molecular mass of approximately 20 kD. The protein migrates at approximately 20 kD in DTT-reducing conditions and approximately 18 kD in non-reducing condition by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity	>98%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in PBS, pH 7.4.
Endotoxin Level	Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration	10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

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BD, 554006, Purified Mouse Anti-Armenian and Syrian Hamster IgG1 G94-56

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