BD, 554147, Purified Mouse Anti-p53 PAb 122

The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers.1 Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and it forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. p53 migrates at a reduced molecular weight of 53 kDa. PAb 122 recognizes mammalian p53, including mouse, rat, hamster, monkey and human p53.2 Cells from the SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4) were used as immunogen. PAb 122 was originally evaluated by ELISA, immunoprecipitation, radioimmunoassays and indirect immunofluorescence of cultured cells.
Applications include immunoprecipitation (1-2 µg/1x10e6 cells), western blot analysis (2 µg/ml), immunofluorescence microscopy of cultured cells and flow cytometry. COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651) or another SV40-transformed cell line are suggested as positive controls for detecting p53. COS-7 cell lysate is optimized for a western blot control and comes ready to load on an SDS-PAGE gel.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.