BD, 555004, Biotin Hamster Anti-Rat CD29 Ha2/5
0.5 mg
Catalog Number: 555004
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Category
Pack Size106 units / pack
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| Source | Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. IgG-Fc-6His tag is located at the C-terminal. |
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| Molecular Mass | The 397 amino acid recombinant protein has a predicted molecular mass of approximately 44.7 kD. The DTT-reduced and non-reduced protein migrate at approximately 55 - 60 kD and 120 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Arg. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in 10 mM Sodium Phosphate buffer, 300 mM NaCl, pH 7.2. |
| Endotoxin Level | Less than 0.1 ng per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | ED50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 106 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Distribution | Activated human T cells, B cells, malignant human lymphoid cell lines, fibroblasts, dendritic cells, thymocytes, macrophages, cardiomyocytes, hepatocytes. |
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| Function | Plays a role in apoptotic clearance of virus-infected cells and contributes to the shutdown of chronic immune responses and to maintenance of peripheral tolerance. Soluble FAS modulates cell apoptosis. |
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| Interaction | Lymphocytes T (mainly CD8+), NK cells. |
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| Ligand/Receptor | TNFLSF6 (FASL). |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Stem Cells |
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| Molecular Family | Cytokine/Chemokine Receptors |
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| Antigen References | 1. Oehm A, et al. 1992. J. Biol. Chem. 267:10709. 2. Cheng J, et al. 1994. Sciences 263:1759. 3. Papoff G, et al. 1996. J. Immunol. 156:4622. 4. Rescigno M, et al. 2000. J. Exp. Med. 192:1661. 5. Giroux M, et al. 2005. Blood 105:703. 6. Kovacic N, et al. 2010. Expert Opin. Ther. Targets 14:1121. |
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| Gene ID | 355 |
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| UniProt | View information about FAS on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Tumor necrosis factor receptor superfamily member 6 (TNFRSF6), ALPS1A, APO-1, Apoptosis antigen 1 (APT1), CD95, FAS1, FASTM, FS-7-associated surface antigen |
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