Description
Vascular endothelial growth factor (VEGF) is a heparin-binding, dimeric protein related to the PDGF/sis family of growth factors. Major sources of VEGF include pituitary cells, monocytes/macrophages, smooth muscle, and keratinocytes. VEGF is a mitogen for endothelial cells, activates and is chemoattractant for monocytes, enhances blood vessel permeability, and is a pro-coagulant. Human VEGF occurs in several molecular variants arising by alternative splicing of the mRNA. The splice forms of VEGF differ in biological properties. VEGF is a homodimeric heavily glycosylated protein of 46-48 kDa (18-25 kDa subunits). Glycosylation is not required, however, for biological activity. The subunits are linked by disulphide bonds. Different isoforms of VEGF have different properties in vitro and this may apply also to their in vivo functions.
IF/IHC: The G153-694 antibody is useful for immunohistochemical staining. Following Retrievagen A pretreatment, purified G153-694 antibody should be used at 2.5 µg/ml to 5 µg/ml and titrated for optimal indirect immunohistochemical staining. Tissues can be visualized via a three-step staining procedure in combination with Biotin Goat anti-Mouse Ig (Cat. No. 550337) secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). More conveniently, the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011) that contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate can be used for staining. Additional protocol information can be found at http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp IP/WB: The purified G153-694 antibody has been reported to be useful to immunoprecipitate native human VEGF and to identify VEGF by Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen. Investigators are advised to determine optimal concentrations for individual applications.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
