The B33-2 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the B33-2 hybridoma was affinity-purified, recombinant human IL-2 protein.
ELISA Detection: Biotinylated B33-2 antibody (Cat. No. 555040) is useful as a detection antibody in sandwich ELISA for measuring human IL-2 protein levels. The biotinylated B33-2 antibody can be paired with purified MQ1-17H12 antibody (Cat. No. 554563) as the capture antibody and with recombinant human IL-2 (Cat. No. 554603) as the standard. Biotinylated B33-2 antibody should be titrated (0.5 - 2.0 µg/mL) to determine the optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of human IL-2 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For serum or plasma, the BD OptEIA™ Human IL-2 ELISA Set (Cat. No. 555190) or BD OptEIA™ Human IL-2 ELISA Kit II (Cat. No.550611) are recommended. This ELISA pair has been reported to show no cross-reactivity with the following cytokines: mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL- 10, IL-12p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2 MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL- 2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This product is manufactured and sold under license from Pestka Biomedical Laboratories, Inc. (d/b/a PBL InterferonSource) and may be used solely as indicated. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics is strictly prohibited. This product is covered by U.S. Patent No. 5,597,901 and Bulgarian Patent No. BG1895.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
