BD, 555072, Purified Mouse Anti-Rat MCP-1 C4

The C4 antibody reacts with rat monocyte chemoattractant protein 1 (MCP-1). The C4 antibody shows no crossreactivity with rat MIP-1α, MIP-1β, RANTES, MIP-2 or KC. The immunogen used to generate this hybridoma was recombinant rat MCP-1 protein.
ELISA Capture: The purified C4 antibody (Cat. No. 555072) can be used as the capture antibody in a sandwich ELISA for measuring rat MCP-1 protein levels in conjunction with the biotinylated B4 antibody (Cat. No. 555074) as the detecting antibody and recombinant rat MCP-1 protein (Cat. No. 555110) as the standard. This purified capture antibody should be titrated in the range of 2 to 10 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of rat MCP-1 protein ranging from ~2,000 to 5 pg/ml should be used. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook. This ELISA pair shows no cross-reactivity with rat MIP-1α, MIP-1β, RANTES, MIP-2 or KC. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring MCP-1 in serum or plasma the rat MCP-1 BD OptEIA™ ELISA Set, Cat. No. 555130, is specially formulated and recommended. IP/WB: The C4 antibody has been reported to be useful for immunoprecipitation and Western blot. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.