BD, 555399, APC Mouse Anti-Human CD14 M5E2
100 Tests
Catalog Number: 555399
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| Brand | BD Pharmingen™ |
|---|---|
| Reactivity | Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog (Tested in Development) |
| Isotype | Mouse IgG2a, κ |
| Immunogen | Human CD14 Protein |
| Application | Flow cytometry (Routinely Tested) |
| Vol. Per Test | 20 µl |
| Workshop Number | II M34; III M329 |
| RRID | AB_398596 |
| Storage Buffer | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
| Regulatory Status | RUO |
Answer:The APC-conjugated monoclonal antibody recognizes human CD14 and uses clone M5E2.
Answer:The nominal staining volume is 20 µL per test; the appropriate staining conditions should be confirmed for the assay.
Answer:A flow cytometer with suitable APC excitation and detection, together with appropriate compensation or spectral controls, is required.
The BD Pharmingen APC-conjugated mouse anti-human CD14 monoclonal antibody, clone M5E2, is designed for flow-cytometric characterization of human monocyte populations. CD14 is a cell-surface glycoprotein commonly expressed at high levels on monocytes and associated with responses to lipopolysaccharide. The allophycocyanin (APC) fluorochrome supports detection in an instrument configured with an appropriate red excitation laser and emission collection channel. Use the antibody to distinguish CD14-positive events in panels that also include lineage, viability and other myeloid markers. The nominal reagent volume is 20 µL per test, although optimal staining should be established under the laboratory's validated workflow. Include compensation or spectral-unmixing controls, an unstained control and suitable gating controls when building a multicolor experiment. Protect the conjugate from excessive light exposure, store refrigerated and do not freeze. This reagent is for research flow cytometry and is not an unconjugated primary antibody.
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