BD, 557702, Alexa Fluor® 488 Mouse IgG1 κ Isotype Control MOPC-21 (also known as MOPC21; MOPC 21)

Name: Alexa Fluor® 488 Mouse IgG1 κ Isotype Control MOPC-21 (also known as MOPC21; MOPC 21)
Brand: BD
SKU: 557702

100 Tests

Catalog Number: 557702

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Category

Amino Acids

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
Recommended Assay Procedure: Immunofluorescent Staining and Flow Cytometric Analysis: The FITC-, PE-, APC-, PE-Cy7-, Alexa Fluor® 488-, and Alexa Fluor® 647-MOPC-21 immunoglobulins (Cat. No. 554679, 559320, 554681, 557646, 557721, and 557732) are suitable mouse IgG1κ isotype controls for assessing the level of background staining on paraformaldehyde fixed/saponin-permeabilized mouse or human cells for flow cytometric analysis.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
8. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.

Specifications

General

Source	Human Cathepsin B, amino acids (Arg18 -Ile339) (Accession# NP_001899.1), with a C-terminal 10-His tag was expressed in 293E cells.
Molecular Mass	The 332 amino acid recombinant protein has a predicted molecular mass of approximately 37 kD. The DTT-reduced and non-reduced protein migrate at approximately 45 kD by SDS-PAGE. The predicted N-terminal amino acid is Arg.
Purity	>95%, as determined by Coomassie stained SDS-PAGE under reducing conditions
Formulation	0.22µm filtered protein solution is in 20 mM Tris, 100 mM NaCl, and pH 8.0.
Endotoxin Level	Less than 0.01ng per µg cytokine, as determined by the LAL method.
Concentration	10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored at -20°C for one month, or at -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.

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