BD, 557735, Alexa Fluor® 647 Rat Anti-Mouse IFN-γ XMG1.2

Name: Alexa Fluor® 647 Rat Anti-Mouse IFN-γ XMG1.2
Brand: BD
SKU: 557735

0.1 mg

Catalog Number: 557735

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Category

Flow Cytometry Buffers

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The XMG1.2 monoclonal antibody specifically binds to mouse interferon-γ (IFN-γ) protein. IFN-γ is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-γ production is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. An isotype control should be used at the same concentration as the antibody of interest.
8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Specifications

General

Brand	BD Pharmingen™
Alternative Name	Ifg; Ifng; IFN-γ; IFN-g; IFN-gamma; Interferon gamma; Type II Interferon
Reactivity	Mouse (QC Testing)
Isotype	Rat IgG1, κ
Immunogen	Mouse IFN-γ Recombinant Protein
Application	Intracellular staining (flow cytometry) (Routinely Tested)
Concentration	0.2 mg/ml
RRID	AB_396843
Storage Buffer	Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status	RUO

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