BD, 558360, Purified Mouse anti-PDGFRβ (CD140b) (pY857) J24-425

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRα (CD140a) and/or PDGFRβ (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to αα PDGFR, PDGF-AB binds to both αα and αβ PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-γ. The J24-425 monoclonal antibody recognizes the phosphorylated tyrosine 857 (pY857) in the tyrosine kinase domain 2 of CD140b, which is required for maximal receptor kinase activity. The orthologous phosphorylation site in mouse PDGFRβ is Y856.

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.