BD, 558708, APC Mouse anti-Human CD212 2.4E6 (also known as 2-4E6)
100 Tests
Catalog Number: 558708
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Pack Size106 units / pack
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| Source | Human FGF-9, amino acids (Met1-Ser208) (Accession# NP_002001.1), was expressed in E. coli. |
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| Molecular Mass | The 208 amino acid recombinant protein has a predicted molecular mass of approximately 23 kD. The DTT-reduced and non-reduced protein migrate at approximately 23 kD by SDS-PAGE. The predicted N-terminal amino acid is Met. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in 20 mM Tris, pH 8.0, 10% glycerol, 100 mM NaCl, and 1% CHAPS. |
| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | The ED50 is 0.2 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 5.0 x 106 units/mg, as determined by a dose-dependent stimulation of NIH3T3 cell proliferation without heparin added to the culture. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Growth factor. |
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| Distribution | Expression of FGF-9 has been reported in lung, γδ T cells, central nervous systems, the epithelium of tooth germs, and the prostate epithelium. |
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| Function | FGF-9 is involved in embryonic development and many biological functions at adulthood. FGF-9 is induced by PGE2. |
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| Interaction | Leydig cells, intestinal mesenchymal stem cells, lung mesenchymal cells, cranial mesenchymal cells, glial cells, fibroblasts, and epithelial cells. |
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| Ligand/Receptor | FGF-9 binds and activates the IIIc isoforms of FGFR1, FGFR2, and FGFR3 and the IIIb isoform of FGFR3. |
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| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells |
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| Biology Area | Cell Biology, Neuroscience, Stem Cells, Synaptic Biology |
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| Molecular Family | Cytokines/Chemokines, Growth Factors |
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| Antigen References | 1. Hendrix ND, et al. 2006. Cancer Res. 66:1354. 2. Colvin JS, et al. 2001. Cell 104:875. 3. Colvin JS, et al. 2001. Development 128:2095. 4. Zhang X, et al. 2006. J. Biol. Chem. 281:15694. 5. Gay D, et al. 2013. Nat. Med. 7:916. 6. Harada M, et al. 2009. Nat. Genet. 41:289. 7. Lai MS, et al. 2014. PLoS One 9:e90243. 8. Barak H, et al. 2012. Dev. Cell 22:1191. 9. Santos-Ocampo S, et al. 1996. J. Biol. Chem. 271:1726. 10. Geske M, et al. 2008. Development 135:2959. |
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| Gene ID | 2254 |
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| UniProt | View information about FGF-9 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Fibroblast Growth Factor 9, Glia-Activating Factor (GAF), Heparin-Binding Growth Factor 9 (HBGF-9), HBFG-9, SYNS3 |
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