BD, 559048, Purified Mouse Anti-Human CD57 HNK-1 (also known as HNK1)

Anti-HNK-1 monoclonal antibody recognizes a 110 kD glycoprotein (CD57) that is expressed on a subset of natural killer (NK) lymphocytes and T lymphocytes. It also recognizes epitopes on neural or neuroectodermal tumors including neurofibromas, malignant melanomas, malignant peripheral neuroectodermal tumors, Ewing's sarcomas, and small-cell lung carcinomas. Anti-HNK-1 monoclonal antibody also recognizes a carbohydrate epitope on subpopulations of several cell-adhesion molecules including N-CAM and myelin-associated glycoprotein (MAG).
Functional Studies: Anti-HNK-1 monoclonal antibody recognizes a subset of NK lymphocytes and T lymphocytes. Unlike the populations of cells recognized by Anti-N-CAM monoclonal antibodies that share the unique ability to mediate direct, non-major histocompatibility complex-restricted cytotoxicity against certain tumor cells, the T lymphocytes recognized by anti-HNK-1 monoclonal antibody do not have NK activity. Anti-HNK-1 monoclonal antibodies can be used with other anti-N-CAM monoclonal antibodies to better understand the role of HNK-1 and N-CAM in leukocyte cytolytic activity against tumor cells. Anti-HNK-1 monoclonal antibody can be used in studies on the role of N-CAM and HNK-1 in the development and regulation of neuroendocrine tissues and muscle regeneration. Immunohistology: Anti-HNK-1 monoclonal antibody can be used to stain paraffin-embedded tissues. Immunoblotting: Anti-HNK-1 monoclonal antibody can be used in Western blot analysis.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.