Description
The C40-1457 monoclonal antibody specifically recognizes CD271 that is also known as the nerve growth factor receptor (NGFR). CD271 is 75 kDa type I transmembrane glycoprotein likewise known as TNFRSF16 that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD271 has been found localized to neuronal axons, Schwann cells, and perineural cells of peripheral nerves. It is also expressed by some epithelial, mesenchymal and lymphoid tissues. NGFR is the receptor for nerve growth factor (NGF), a polypeptide that is essential for normal development of the nervous system. NGF promotes survival and differentiation of sympathetic and sensory neurons during embryological development of the peripheral nervous system. NGF binds to two distinctive surface receptors, the p/140[prototrk] and p75[NGFR]. High affinity binding of NGF requires that both receptor molecules be expressed. NGFR is expressed on human and rat lymphocytes. A subset of lymphoid cells in the spleen, lymph nodes, and follicular dendritic cells in germinal centers of reactive lymph nodes were found to express CD271. It has been reported that NGFR interaction with its ligand, NGF, may play a role in immunoregulation. NGF may also function as a B-cell growth factor.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
8. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
