Description
The BD CBA Human Soluble CD14 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human Soluble CD14 Flex Set will be 10,000 pg/ml. An example standard curve is shown in Figure 1. Due to high levels of sCD14 in serum and plasma samples, a starting dilution of 1:200 is recommended. The BD CBA Human Soluble CD14 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set or CD Marker Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets) nor with any BD CBA Human Soluble Receptor Protein Flex Set reagents. For technical protocols and additional resources, please visit "Bead-Based Immunoassays" at our website: http://www.bdbiosciences.com/us/s/resources. Performance Limit of Detection: The theoretical limit of detection is 9.2 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.
1. ProClin is a trademark of Rohm and Haas Company.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Licensed for Research Purposes Only. Commercial use requires license from Boyce Thompson Institute for Plant Research.
5. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
6. Hazardous Ingredient: ProClin™ 150. Avoid exposure to skin and eyes and ingestion. Wash exposed skin with soap and water. Flush eyes with water.
