BD, 561016, Alexa Fluor® 700 Mouse anti-Human Granzyme B GB11
25 µg
Catalog Number: 561016
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| Source | Biotinylated recombinant human SIRPA, amino acid (Glu31-Arg370) (Accession # P78324.2), with a linker, a C-terminal Human IgG (Pro100-Lys330), and an Avi-tag was site-specifically biotinylated by the enzyme BirA, was expressed in 293E. |
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| Molecular Mass | The 592 amino acid recombinant protein has a predicted molecular mass of approximately 65.8 kD. The DTT-reduced protein migrates at approximately 75 - 115 kD, and the non-reduced protein migrates at approximately 140 – 240 kD by SDS-PAGE. The predicted N-terminal amino acid is Glu. |
| Purity | > 95%, as determined by Coomassie-stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, 5 % Glycerol, pH 7.4. |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | When recombinant human CD47 (Cat. No. 785406) is immobilized at 1 µg/mL, biotinylated recombinant human SIRPα binds in a dose-dependent manner with an ED50 range of 4 – 24 ng/mL. HRP-Avidin (Cat. No. 405103) was used to detect the binding. |
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| Application | Biossay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Disulfide-linked homodimer |
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| Distribution | Broadly distributed in normal adult tissues including brain, endocrine tissues, bone marrow/immune system, lung, pancreas, gastrointestinal tract, kidney, testis, and ovary |
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| Function | An inhibitory receptor is present in myeloid cells. It interacts with the widely distributed membrane protein CD47 to prevent the phagocytosis of healthy cells. |
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| Interaction | Interacts with CD47-expressing cells throughout the body |
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| Ligand/Receptor | CD47 |
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| Bioactivity | Measured by immobilized recombinant human CD47 binding biotinylated recombinant human SIRPα in a dose-dependent manner. |
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| Cell Type | Macrophages, Neurons |
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| Biology Area | Cell Adhesion, Innate Immunity, Neuroscience |
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| Molecular Family | Adhesion Molecules, Soluble Receptors |
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| Antigen References | van Beek EM, et al. 2005. J Immunol. 12:7781-7. Lee WY, et al. 2007. J Immunol. 11:7741–50. Subramanian S, eet al. 2006. Blood. 107: 2548-56. Barclay AN. 2009. Current Opinion in Immunology. 21:47-52. Liu Y, et al. 2005. J Biol Chem. 43:36132-40. |
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| Gene ID | 140885 |
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| UniProt | View information about SIRPalpha on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Human SIRPa, SI, SIRPA, SIRPalpha, CD172a, BIT, MacrophageFusion Receptor, MFR, P84, MYD-1, SHP substrate 1, SHPS1, PTPNS1 |
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