BD, 561604, APC Mouse Anti-Human CD7 M-T701
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Catalog Number: 561604
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| Source | Recombinant human CA9, amino acid Pro59-Asp414 (Accession No. Q16790) was expressed in CHO cells. |
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| Molecular Mass | The 365 amino acid recombinant protein has a predicted molecular mass of approximately 42.5 kD. The DTT-reduced protein migrates at approximately 50 kD and non-reduced protein migrates at approximately 100 kD by SDS-PAGE. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in 25mM Tris, 150mM NaCl, pH 7.5 |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | The esterase activity of CA9 is used to measure its specific activity (SA). The SA is > 20 pmole/min/µg, at an absorbance of 400 nm with 2 mM of enzymatic substrate (4-Nitrophenyl acetate) and 2 µg enzyme in 50 µL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
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| Application | Bioassay |
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| Application Notes | The esterase activity of Human Carbonic Anhydrase 9 (hCA9) activity is used to measure its specific activity (SA). The SA is > 20 pmole/min/μg, at an absorbance of 400 nm with 2 mM of enzymatic substrate (4-Nitrophenyl acetate) and 2 μg enzyme in 50 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The increase of the product is monitored by increase in intensity of fluorescence at 400 nm. Materials 1. Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5 2. Recombinant Human Carbonic Anhydrase 9 3. 2 mM of enzymatic substrate (4-Nitrophenyl acetate) and 2 μg enzyme in 50 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. 4. 4-Nitrophenol Activity assay procedures 1. Prepare hCA9 to 40 μg/mL in Assay Buffer 2. Dilute 4-Nitrophenol to 0.4 mM in assay buffer. 3. Add 100 µL of the 0.4 mM 4-Nitrophenol or 40 μg/mL hCA9 into the top concentration well 4. In a 96-well black/clear bottom plate, prepare in 1:2 serial dilutions of 4-Nitrophenol or hCA9 in assay buffer, 50 μL/well, with the top concentration of 0.4 mM (2X) 4-Nitrophenol or 40 μg/ml (2X) hCA9 Dilute substrate to 2 mM in Assay Buffer 5. Read the product formation by measuring at 400 nm in kinetic mode for 5 minutes. 6. Calculate specific activity using the equation below: * Adjusted for substrate blank **Derived using calibration standard 4-Nitrophenol Per well Recombinant human CA9: 1 µg Substrate: 1 mM BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomor |
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| Distribution | A431 human epithelial carcinoma cells line, U87 MG human glioblastoma/astrocytoma cell line, cancer cells |
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| Function | CA9 interconverts carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. HIV-1 infection (VSV-G pseudotyped) of CEMT4 T cells down-regulates plasma membrane expression of CA9. |
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| Ligand/Receptor | CO2 and H2O |
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| Bioactivity | The esterase activity of CA9 is used to measure its specific activity. |
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| Cell Type | Basophils, Mast cells, Monocytes, Neutrophils |
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| Biology Area | Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | Uda NR, et al. 2015. J Enzyme inhib Med Chem. 6:955-60. Pastorekova S, et al. 2019. Cancer Metastasis Rev. 38:65-77. Pastorek J, et al. 1994. Oncogene. 9:2877-88. Loncaster JA, et al. 2001. Cancer Res. 61:6394-9. Kaluzova, M, et al. 2004. Mol Cell Biol. 24:5757-66. Pan P, et al. 2006. J Physiol. 571:319-27. Zhou GX, et al. 2010. Urology. 75:257-61. |
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| Gene ID | 768 |
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| UniProt | View information about Carbonic Anhydrase 9 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Carbonic anhydrase 9, Carbonate dehydratase IXCA9, CAIX, CA-IX, ,Carbonic Anhydrase IX, carbonic dehydratase, EC 4.2.1.1, G250,Membrane antigen MN, MN, P54/58N, PMW1, RCC, RCC-associatedantigen G250, RCC-associated protein G250 |
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