BD, 562197, Purified Mouse Anti-RORγt O28-835.24.46

The O28-835 monoclonal antibody recognizes ROR-gamma (RORγ) and its isoform, RORγt. RORγ (~58 kDa calculated molecular weight) is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. It is expressed in different tissues including skeletal muscle, kidney, liver and adipose tissues. RORγt (~56 kDa calculated molecular weight) is a RORγ isoform that is expressed exclusively by lymphoid cells. It is expressed by various lymphoid cell types including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells and lymphoid tissue inducer (Lti) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.