BD, 562274, Alexa Fluor® 488 Mouse anti-PDX-1 658A5.1

The 658A5 monoclonal antibody binds PDX-1 or Pancreas/Duodenum Homeobox Protein-1 encoded by the PDX1 or IPF1 (Insulin Promoter Factor 1) gene. PDX-1 is a key regulator of pancreatic development and adult beta-cell function. Loss of PDX1 gene function in mice and humans results in pancreatic agenesis. PDX-1 is also involved in endocrine precursor cell development by binding the DNA-binding transcription factor Neurogenin-3. PDX-1-positive cells have been obtained by the directed differentiation of human embryonic stem cells through a definitive endoderm lineage.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.