Description
The CX5 monoclonal antibody specifically binds to NKG2D, also known as CD314. NKG2D is a lectin-like receptor that is detected on resting and IL-2-activated NK cells, activated CD8-positive T lymphocytes, and LPS-activated macrophages, but not on resting T cells or unstimulated macrophages. NKG2D has little homology to the other members of the NKG2 family, NKG2A, C, and E, and does not form heterodimers with CD94. On NK cells, NKG2D is an activating receptor that associates with DAP10, an adapter protein that stimulates the PI3 kinase pathway. An isoform of mouse NKG2D can also associate with the signaling adapter protein DAP12, also known as KARAP (Killer cell-Associated Receptor-Associated Polypeptide), that activates the Syk and ZAP70 tyrosine kinases. On cytotoxic T cells, NKG2D is a co-stimulatory receptor that associates with DAP10. The ligands for NKG2D include the minor histocompatibility antigen H60, MULT1 (Murine UL16-binding protein-Like Transcript 1), and the five retinoic acid-inducible proteins Rae-1α, β, γ, δ and ε. Interactions of NKG2D with its ligands are involved in the regulation of innate and immune cytotoxic responses to tumor and pathogen-infected cells and in diabetes progression in the NOD mouse. The CX5 mAb blocks the binding of NKG2D to its ligands.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
