Description
The BY55 monoclonal antibody specifically binds to the human cell surface molecule, CD160 (also known as BY55). CD160 is a glycosyolphosphatidylinositol-anchored glycoprotein member of the immunoglobulin superfamily. It is expressed on cytotoxic αβ CD8+ T cells and natural killer (NK) cells, γδ T cells and intestinal intraepithelial T lymphocytes. CD160 can bind to classical and nonclassical MHC class I molecules. This interaction can costimulate the proliferation of activated human T lymphocytes including CD28-negative T lymphocytes.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
8. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
9. Cy is a trademark of GE Healthcare.
10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
