Description
The 1H7 monoclonal antibody specifically binds to the α subunit of the human insulin-like growth factor-I (IGF-I) receptor that is also known as CD221. CD221 is a receptor tyrosine kinase that has high affinity for IGF-1 and low affinity for insulin and IGF-2. CD221 is synthesized as a 180 kDa precursor molecule that is processed into two subunits, a 135 kDa α subunit which is disulfide-linked to a 90 kDa β subunit. The mature IGF-IR complex is comprised of two extracellular α subunits and two transmembrane β subunits that each possesses a tyrosine kinase domain. When bound by ligand, the IGF-1 receptor autophosphorylates multiple tyrosine sites in its β chains and phosphorylates other signaling molecules that regulate cell growth, development, and neoplastic transformation. CD221 is structurally and functionally similar to the insulin receptor (aka, CD220) and is expressed on a variety of human hematopoietic and non-hematopoietic cells. The 1H7 antibody does not crossreact with the human insulin receptor and can block IGF-I- or IGF-II-stimulated cell growth.
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
