Description
The 6C2 monoclonal antibody specifically binds to the mouse Toll like receptor 2 (TLR2) that is also known as CD282. CD282 is a type 1 transmembrane glycoprotein and a TLR family member. It is expressed on the surface of monocytes, macrophages, dentritic cells, granulocytes, endothelial and epithelial cells. This pattern recognition receptor is important for sensing and transducing signals in response to microbial cell wall components including lipoproteins, peptidoglycans and lipoteichoic acid. CD282 signals through intracellular MYD88 and TRAF6 leading to NF-kappa-B activation and the production of cytokines and inflammatory mediators by responding cells. TLR2 plays a major role in mediating inflammatory and innate immune responses.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
7. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
8. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
9. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
