BD, 563490, Alexa Fluor® 647 Mouse Anti-PLZF R17-809.44.19

The R17-809 monoclonal antibody recognizes promyelocytic leukemia zinc finger protein (PLZF). PLZF is also known as zinc finger and BTB domain containing 16 (ZBTB16) and Zinc finger protein 145. PLZF is a member of the BTB/POZ-ZF family of transcription factors that includes Th-POK (Zbtb7b) which is involved in CD4+ T cell fate determination. PLZF serves multiple functions. In the immune system, PLZF is involved in the developmental regulation of innate immune lymphocytes including NKT cells, a subset of γδ T cells, and mucosal associated invariant T (MAIT) cells. The R17-809 hybridoma was generated from a mouse immunized with recombinant mouse PLZF protein. The R17-809 antibody crossreacts with both mouse and human PLZF.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.