Description
The TU39 monoclonal antibody specifically recognizes human major histocompatibility (MHC) Class II HLA-DR, DP and most DQ antigens. These antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6. MHC Class II antigens are transmembrane heterodimeric glycoproteins composed of α chain (36 kDa) and β chain (27 kDa) subunits. They are expressed primarily on antigen presenting cells which include dendritic cells, monocytes, macrophages, thymic epithelial cells, and B cells. They are also expressed on activated T cells. This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4+ T lineage cells. The TU39 antibody is reportedly useful for immunophenotyping as well as functional studies including the inhibition of mixed lymphocyte reactions and antibody-mediated complement fixation on target cells.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
