Description
The Q21-559 monoclonal antibody recognizes ROR gamma t (RORγt), an isoform of ROR gamma (RORγ). RORγt is a DNA-binding transcription factor that is encoded by the RORC (RAR-related orphan receptor C) gene. It belongs to the ROR/RZR orphan nuclear receptor family. Two protein isoforms are produced from the RORC gene by alternative splicing: RORγ or RORγt. The RORγ isoform is expressed in many tissues, including thymus, lung, liver, kidney, muscle, and brown fat. However the tissue distribution of the second isoform RORγt is highly restricted to the thymus where it is expressed exclusively in immature CD4+CD8+ thymocytes and in peripheral Th17 and LTi cells. RORγt reportedly functions as an essential transcription factor for the development of secondary lymphoid tissues. It also plays an important regulatory role in thymopoiesis and effector T cell differentiation, especially for Th17 cells.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
6. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
8. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
