Description
The O21-404 monoclonal antibody recognizes the human mammalian target of rapmycin,(mTOR) that is phosphorylated at serine residue 2448, mTOR (pS2448). mTOR belongs to the phosphoinositide-3-kinase (PI3K)-related (PIKK) family of kinases. mTOR is also known as mechanistic target of rapamycin (serine/threonine kinase), FRAP, RAFT1, and RAPT1. mTOR functions as an amino acid and ATP sensor to balance nutrient availability and cell growth. When nutrients are sufficiently available, mTOR is activated by phosphorylation at serine residue 2448 through the PI3 kinase/Akt signaling pathway. Phosphorylated mTOR in turn activates the p70 S6 kinase and contributes to the inactivation 4E-BP1, an elF4E inhibitor. In this way, mTOR participates in the upregulation of cellular protein synthesis, growth, proliferation and survival. mTOR function may be abnormally regulated in tumors.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
7. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
