BD, 564314, Alexa Fluor® 647 Mouse Anti-Human CD49b AK-7 (also known as AK7)
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Catalog Number: 564314
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| Source | Recombinant human IL-3, amino acid sequence containing Ala20-Phe152 (Accession No. NP-000579), was expressed in E.coli. The carboxy-terminus contains a linker-6His tag. |
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| Molecular Mass | The 146 amino acid recombinant protein has a predicted molecular mass of approximately 16.24 kD. The DTT-reduced and non-reduced proteins migrate at approximately 16 kD - 18 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala. |
| N-terminalSequence Analysis | Met-Ala-Pro-Met-Thr-Gln-Thr-Thr-Ser-Leu |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.22 μm filtered protein solution is in PBS, pH 6.5. |
| Endotoxin Level | Less than or equal to 0.1 EU per μg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/μg by ELISA |
| Concentration | 25 μg and 100 μg sizes are bottled at 0.5 mg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Recombinant human IL-3 induces the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.25 – 1.25 ng/mL. The specific activity of Cell-Vive™ GMP Recombinant Human IL-3 (carrier-free) is ≥ 1.6 x 105 IU/mg when compared against the WHO International Standard for human IL-3 (NIBSC code: 91/510). |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Monomer |
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| Bioactivity | Measured by its ability to induce the proliferation of TF-1 human erythroleukemic cells. |
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| Cell Sources | Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells |
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| Cell Targets | Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages |
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| Receptors | Heterodimeric receptor formed by IL-3Rα (CD123) and a common β subunit (βc) that is shared with the IL-5R and GM-CSFR |
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| Antigen References | Kitamura T, et al. 1989. J Cell Physiol. 140:323-34. Kohno M, et al. 2005. Blood. 105:2059-65 Murphy JM and Young IG. 2006. Vitam Horm. 74:1-30. Kleemann R, et al. 2008. Cardiovasc Res. 79:360-76. Valent P and Dahinden CA. 2010. Curr Opin Hematol. 17:60-6. Mirza S, et al. 2010. J Biol Chem. 285:2237. Bénard A, et al. 2021. Nat Commun. 12:1112. |
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| Regulation | IL-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections. |
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| Gene ID | 3562 |
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| UniProt | View information about IL-3 on UniProt.org |
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| Other Names | Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor |
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