BD, 564679, BB515 Mouse Anti-Human CD66b G10F5

Name: BB515 Mouse Anti-Human CD66b G10F5
Brand: BD
SKU: 564679

100 Tests

Catalog Number: 564679

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Category

Fluorescent-Conjugated Antibodies for FACS

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The G10F5 monoclonal antibody specifically binds to CD66b, also known as Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8). CD66b is a glycosylphosphatidylinositol (GPI) linked protein with a molecular weight of 100 kDa expressed on granulocytes. This molecule was previously clustered as CD67 in the Fourth Human Leucocyte Differentiation Antigen (HLDA) Workshop and renamed CD66b in the Fifth HLDA Workshop. CD66b is a member of the carcinoembryonic antigen (CEA)-like glycoprotein family present on granulocytes and referred to as non-specific crossreacting antigens (NCA). Granulocyte activation induced with soluble stimulators (calcium ionophore, phorbol myristate acetate, N-formylmethionyl- leucyl-phenylalanine) results in release and increased expression of NCA. Findings suggest that these molecules may play a role in phagocytosis, chemotaxis and adherence.
For optimal results, it is recommended to perform 2 washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescent staining, prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BB515 under optimum conditions and unconjugated antibody was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. Diagnostic uses require a separate license.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Specifications

General

Brand	BD Horizon™
Alternative Name	CEACAM8; CGM6; NCA-95
Reactivity	Human (QC Testing)
Isotype	Mouse BALB/c IgM, κ
Immunogen	Human Granulocytes
Application	Flow cytometry (Routinely Tested)
Vol. Per Test	5 µl
Workshop Number	V 5T-127, MA020
RRID	AB_2738889
Storage Buffer	Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status	RUO

FAQs

Question: What cells were used as the immunogen?

Answer:Clone G10F5 was generated against human granulocytes.

Question: How much reagent is used per test?

Answer:Use 5 µL per test, typically with 1 × 10^6 cells in a 100 µL sample.

Question: How should stained cells be washed?

Answer:Wash the cells twice with wash buffer before acquisition to limit spread of the negative population.

Application Note

BB515 Mouse Anti-Human CD66b, Clone G10F5 is used for flow-cytometric detection of human CD66b, also known as CEACAM8, CGM6, or NCA-95. The monoclonal antibody has a mouse BALB/c IgM, κ isotype and was generated against human granulocytes. Human reactivity receives quality-control testing, and flow cytometry is routinely tested. The antibody is affinity purified from tissue-culture supernatant or ascites and directly conjugated with BD Horizon BB515; unconjugated antibody is removed after labeling. Direct fluorescence avoids a secondary-antibody incubation and supports single-color or multicolor immunophenotyping. The reagent arrives prediluted at 5 µL per test, with a typical test defined as 1 × 10^6 cells in a 100 µL sample. After staining, wash the cells twice with wash buffer before flow-cytometer acquisition. Two washes help limit spreading of the negative population that can increase when fewer wash steps are used. Use an isotype control at the same concentration when the panel requires control of nonspecific staining. The aqueous buffered formulation contains no more than 0.09% sodium azide. Store it undiluted at 4 °C, protect it from prolonged light exposure, and do not freeze. The combination of a directly attached BB515 dye, fixed per-test volume, routinely tested application, and a defined two-wash workflow supports consistent identification of CD66b-positive populations in human research samples. The product is for research use only.

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