BD, 564806, Alexa Fluor® 647 Mouse Anti-Human HLA-DQ Tu169
100 Tests
Catalog Number: 564806
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| Source | Recombinant Human L1CAM/CD171, amino acid Ile20-Glu1120 (Accession No. NM_024003.2) and a C-terminal Fc tag was expressed in 293E cells. |
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| Molecular Mass | The 1338 amino acid recombinant protein has a predicted molecular mass of approximately 149.83 kD. The DTT-reduced protein migrates at approximately 210 - 225 kD and non-reduced protein migrates at approximately 250 - 300 kD by SDS-PAGE. The predicted N-terminal amino acid is IIe. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | ED50 = 0.8 - 8 µg/mL as measured by the ability of immobilized protein to support the adhesion of Neuro-2a cells. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | It contains several immunoglobulin-like domains and fibronectin-like repeats (type III), linked via a single transmembrane sequence to a conserved cytoplasmic domain. |
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| Distribution | Tetanus-toxin positive neurons, endothelial cells, certain epithelial cells, reticular fibroblasts, colon and breast carcinomas, colon melanoma, tumor cells of neuronal and mesothelial origin |
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| Function | Homotypic and heterotypic cell-cell interaction in neuronal myelination, neurite outgrowth, regeneration, axonal guidance, and neuronal migration |
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| Interaction | Endothelial cells, epithelial cells, fibroblasts, neurons |
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| Ligand/Receptor | Homophilic interaction with itself and heterophilic binding to integrins, other cell adhesion molecules, extracellular matrix molecules |
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| Bioactivity | Measured by its ability to support the adhesion of neuro-2a cells |
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| Cell Type | Endothelial cells, Epithelial cells, Fibroblasts, Neurons |
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| Biology Area | Cancer Biomarkers, Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Oswald J, et al. 2021. Mol Ther Methods Clin Dev. 21:180-198. Ng PK, et al. 2018. Cancer Cell. 33:450-462. Weinberger V, et al. 2019. Front Oncol. 9:265. Karnezis AN, et al. 2017. J Pathol Clin Res. 3:279-293. Er EE, et al. 2018. Nat Cell Biol. 20:966-978. Samatov TR, et al. 2016. Progress in Histochemistry and Cytochemistry. 51:25-32. |
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| Gene ID | 3897 |
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| UniProt | View information about L1CAM/CD171 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | antigen identified by monoclonal R1, CAML1, CAML1N-CAML1, CD171 antigen, CD171, L1CAM, MASA, MIC5, N-CAM-L1, NCAML1, neural cell adhesion molecule L1, Cell Adhesion Molecule L1, L1 cell adhesion molecule, S10, SPG1 |
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