Description
The 11C1 monoclonal antibody recognizes CD268 which is also known as the B cell activating factor receptor (BAFF-R). CD268 is a type III transmembrane protein of approximately 184 residues. BAFF-R is one of the receptors for B-cell activating factor (BAFF), a member of the TNF family of proteins. BAFF is a key regulator for B cell differentiation and critical in regulating survival and activation of peripheral B cell populations. Experiments in the mouse model show that interaction of BAFF with BAFF-R promotes NF-κB activity. Overexpression of BAFF results in an expanded B cell compartment and autoimmunity in mice. Mice injected with BAFF-neutralizing-Fc (BAFF-R-Fc) protein showed reduced NF-κB activation, blocking BAFF-induced B cell proliferation. Reports suggest that the BAFF-BAFF-R interaction may lead to a better understanding of autoimmune disorders.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
