BD, 565106, APC-R700 Mouse Anti-Human CD25 2A3
100 Tests
Catalog Number: 565106
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| Source | Recombinant human CLEC2D/OCIL, amino acid Arg60 - Val191 (Accession No. Q9UHP7) was expressed in 293E cells. The C-terminal includes TGSR- hIgG1-Fc+6His |
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| Molecular Mass | The 376 amino acid recombinant protein has a predicted molecular mass of approximately 42.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 50 – 62 and 80 – 115 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Arg. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Immobilized recombinant human CLEC2D/OCIL at 2 µg/mL binds to biotinylated recombinant CD161/CLEC5B in a dose-dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.1 – 0.7 µg/mL |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Disulfide-linked homodimer |
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| Distribution | Hematopoietic cell lines, activated lymphocytes (NK cells, T cells, B cells), and antigen presenting cells |
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| Function | Receptor for Killer cell lectin like receptor B1, inhibits osteoclast formation and bone resorption |
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| Interaction | NK cells, T cell, Thymocytes |
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| Ligand/Receptor | CD161 (CLEC5B) |
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| Bioactivity | Measured by its ability to bind CD161/CLEC5B |
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| Cell Type | B cells, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, NK cells, T cells |
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| Biology Area | Immuno-Oncology, Immunology, Innate Immunity |
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| Molecular Family | Innate Immune Signaling |
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| Antigen References | Germain C, et al. 2010. J Biol Chem. 285:36207-15. Rosen DB, et al. 2005. J Immunol. 175:7796-9. Gange CT, et al. 2004. J Biol Chem. 279:29043-9. Hu YS, et al. 2004. J Bone Miner Res. 19:89-99. Lai J, et al. 2020. Immunity. 52:123. Sun Y, et al. 2019. J Cancer Metastasis Treat. 5:80. |
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| Gene ID | 29121 |
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| UniProt | View information about CLEC2D/OCIL on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CLEC2D, C-type lectin domain family 2 member D, C-type lectindomain family 2, member D, C-type lectin related f, Lectin-like NKcell receptor, Lectin-like transcript 1, LLT1, LLT1 Osteoclastinhibitory lectin, OCIL, OCILmember D, CLAXLLT-1 |
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