BD, 565139, APC-R700 Mouse Anti-Human CD56 NCAM16.2 (also known as NCAM 16)
100 Tests
Catalog Number: 565139
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| Brand | BD Horizon™ |
|---|---|
| Alternative Name | NCAM1; NCAM-1; NCAM; Leu-19; Neural cell adhesion molecule 1; NKH1; MSK39 |
| Reactivity | Human (QC Testing) |
| Isotype | Mouse BALB/c IgG2b, κ |
| Immunogen | Immunoaffinity-enriched adult human brain NCAM |
| Application | Flow cytometry (Routinely Tested) |
| Vol. Per Test | 5 µl |
| Workshop Number | V NK60 |
| RRID | AB_2744429 |
| Storage Buffer | Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide. |
| Regulatory Status | RUO |
Answer:The reagent uses clone NCAM16.2 and a mouse BALB/c IgG2b, κ isotype.
Answer:Use 5 µL per test, typically with 1 × 10^6 cells in a 100 µL sample.
Answer:Store undiluted at 4 °C, protect from prolonged light exposure, and do not freeze.
APC-R700 Mouse Anti-Human CD56, Clone NCAM16.2 is used for flow-cytometric detection of human CD56, also known as NCAM1, NCAM, Leu-19, NKH1, or neural cell adhesion molecule 1. The monoclonal antibody has a mouse BALB/c IgG2b, κ isotype and was generated against immunoaffinity-enriched adult human brain NCAM. Human reactivity receives quality-control testing, and flow cytometry is routinely tested. The antibody is affinity purified from tissue-culture supernatant or ascites and directly conjugated with BD Horizon APC-R700. Unconjugated antibody and free fluorochrome are removed, producing a fluorescent primary antibody that avoids a secondary-antibody staining step. The reagent arrives prediluted at 5 µL per test. A typical test contains 1 × 10^6 cells in a 100 µL experimental sample, which supports repeatable setup of human immunophenotyping panels and single-color controls. The aqueous buffered formulation contains BSA, protein stabilizer, glycerol, and no more than 0.09% sodium azide. Store it undiluted at 4 °C, protect it from prolonged exposure to light, and do not freeze it. The NCAM16.2 clone, direct APC-R700 label, routinely tested flow-cytometry application, and fixed per-test volume support consistent CD56-positive population analysis in human research samples. Predilution reduces preparation steps, while the stabilizer-containing buffer maintains the reagent in liquid form under refrigerated storage. The antibody is for research use only and not for diagnostic or therapeutic procedures.
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