Description
The S50-895 monoclonal antibody specifically recognizes the transcription factor, Aiolos, which is encoded by IKZF3, IKAROS family zinc finger 3. Aiolos belongs to the Ikaros family of zinc-finger proteins that includes Ikaros and Helios. Aiolos can form homodimers or heterodimers with other Ikaros family members. Multiple Aiolos isoforms have been described. Aiolos is expressed in committed lymphoid progenitors and mature types of T and B lymphocytes, but is not expressed by hematopoietic stem cells. Aiolos plays an important role in the control of B lymphocyte differentiation and proliferation and is specifically required for the generation of long-lived, high affinity plasma cells in the bone marrow. Aiolos is involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner. Aberrant expression of IKZF3 has been implicated with leukemogenesis and certain autoimmune diseases.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
