Description
The N71-1183 monoclonal antibody specifically recognizes the alpha subunit, also known as p19, of mouse interleukin 23 that is encoded by the Il23a gene. Interleukin 23 (IL-23) is a heterodimeric cytokine composed of a p40 subunit and a p19 subunit. The p19 subunit is unique to IL-23, while the p40 subunit is shared with IL-12 and is also known as IL-12B. Human and mouse p19 share 70% amino acid sequence identity. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4 and TLR8 ligands, as well as the β-glucan receptor, Dectin-1. IL-23 has biological activities that are similar to, but distinct from IL-12: Both IL12 and IL-23 induce proliferation and IFN-γ production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 are restricted to memory T cells. IL-23, but not IL-12, has been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. In vitro and in vivo treatment with N71-1183 monoclonal antibody has been shown to inhibit IL-23's ability to enhance IL-17 production by activated MRL/lpr T lymphocytes (Kyttaris VC, Kampagianni O, Tsokos GC, 2013).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
