BD, 565358, Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control G155-178

Name: Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control G155-178
Brand: BD
SKU: 565358

50 µg

Catalog Number: 565358

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Category

Flow Cytometry Buffers

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The G155-178 clone has an unknown specificity. Trinitrophenol (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to immunoglobulin Fc receptors. The immunoglobulin secreted by the G155-178 hybridoma was selected as a mouse IgG2a, κ isotype control following screening for low background binding on a variety of mouse and human tissues.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Specifications

General

Brand	BD Pharmingen™
Alternative Name	IgG2a kappa Isotype Control
Isotype	Mouse BALB/c IgG2a, κ
Immunogen	TNP-keyhole limpet hemocyanin
Application	Flow cytometry, Isotype control (Routinely Tested)
Concentration	0.2 mg/ml
RRID	AB_2869661
Storage Buffer	Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status	RUO

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