BD, 565546, APC-R700 Rat Anti-Mouse CD200 OX-90.1.1 (also known as OX90)

The OX-90 monoclonal antibody specifically recognizes CD200 (OX-2 antigen), a type-1 transmembrane glycoprotein containing two extracellular Ig-like domains. CD200 is expressed on thymocytes, neurons, B lymphocytes, splenic follicular dendritic cells and endothelium, and subsets of T lymphocytes and dendritic cells; but not on NK cells, granulocytes, monocytes, or macrophages. CD200 Receptor (CD200R; OX2R) expression is restricted to myeloid cells, and it appears that its engagement with CD200 results in inhibition and/or down-regulation of myeloid cell activity.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon APC-R700 under optimum conditions, and unconjugated antibody and free BD Horizon APC-R700 were removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. An isotype control should be used at the same concentration as the antibody of interest.