Description
The ALULA monoclonal antibody specifically binds to Integrin alpha v beta 5 (αvβ5), which is also known as CD51/β5. Integrin αvβ5 is widely expressed by fibroblasts, endothelial cells, and vascular smooth muscle cells. It is involved in activation-dependent cell migration and cell adhesion to the extracellular matrix. Integrin αvβ5 plays a major role in growth factor-induced angiogenesis in response to VEGF and TGF. The αv Integrins, αvβ5 and αvβ3, αre involved in tumor growth and are being targeted in cancer therapies. The ALULA clone specifically recognizes αvβ5 integrin and functionally blocks the interaction between αvβ5 and vitronectin. Although raised against mouse αvβ5, the ALULA antibody crossreacts with human αvβ5.
Investigators: Please note that for optimal results, the target cells should be maintained at 4°C throughout staining and analysis .
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
