BD, 565857, Alexa Fluor® 647 Mouse Anti-Human CD45RC MT2

The MT2 monoclonal antibody specifically binds to CD45RC isoforms of CD45 that share exon 6-encoded sequences. CD45 is a type I transmembrane glycoprotein that serves as a receptor-like protein tyrosine phosphatase (PTP). CD45RC is highly expressed on medullary thymocytes, CD8+ T cells, B cells, NK cells, and by subsets of cortical thymocytes or CD4+ T cells. CD45RC may play a role in regulating receptor-mediated leucocyte signaling responses.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Cy is a trademark of Amersham Biosciences Limited.
7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
8. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
10. An isotype control should be used at the same concentration as the antibody of interest.