Description
The 54/HIF-1α monoclonal antibody specifically recognizes the alpha subunit (HIF-1alpha/HIF-1a/HIF1A) of the heterodimeric helix-loop-helix transcription factor, Hypoxia-induced factor 1 (HIF-1) complex which is involved in cellular O2 homeostasis. Under hypoxic conditions, HIF-1a accumulates and translocates into the nucleus, where it binds to the constitutively-expressed HIF-1 beta subunit (HIF-1b, also known as, Aryl hydrocarbon receptor nuclear translocator/ARNT). This complex can then bind to hypoxic response elements (HREs) of target genes, including glucose transporters, glycolytic enzymes, erythropoietin, vascular endothelial growth factor (VEGF), and various other genes that facilitate metabolic adaptation to hypoxia. HIF-1a is also known to be regulated by proinflammatory cytokines and bacterial products and plays an essential role in inflammation, immunity, and cancer. HIF-1a is rapidly degraded under normoxic conditions.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
