BD, 566010, Alexa Fluor® 647 Mouse Anti-Human S100A8/A9 (MRP-8/14) 5.5

Name: Alexa Fluor® 647 Mouse Anti-Human S100A8/A9 (MRP-8/14) 5.5
Brand: BD
SKU: 566010

50 Tests

Catalog Number: 566010

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Category

Fluorescent-Conjugated Antibodies for FACS

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The 5.5 monoclonal antibody specifically recognizes S100 calcium binding protein A8 (S100A8) and S100A9 (S100A8/A9), which is also known as Migration inhibitory factor-related protein 8 (MRP-8) and MRP-14 (MRP-8/14), Calprotectin, Cystic fibrosis antigen (CF antigen, CFAG) or L1 light and heavy chain. S100A8 and S100A9 are members of the S100 family that regulate myeloid cell functions, inflammation and innate immune responses. S100A8/A9 (MRP-8/14) is expressed in monocytes and neutrophils. Although not generally expressed in tissue macrophages, S100A8/A9 can be detected within some mononuclear cells comprising inflammatory infiltrates. Soluble S100A8/A9 serves as an early serum marker for detecting and monitoring proinflammatory or immunological disease activities.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
9. An isotype control should be used at the same concentration as the antibody of interest.

Specifications

General

Brand	BD Pharmingen™
Alternative Name	S100A8/S100A9; MRP-8/MRP-14; Calprotectin, CFAG; p8,14
Reactivity	Human (QC Testing)
Isotype	Mouse BALB/c IgG1, κ
Immunogen	Acute Monocytic Leukemia Cells and Monocytes
Application	Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test	5 µl
RRID	AB_2732837
Storage Buffer	Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status	RUO

FAQs

Question: Which application is routinely tested?

Answer:Intracellular staining of human samples by flow cytometry is routinely tested.

Question: How much reagent is used per test?

Answer:Use 5 µL per test; a typical test contains 1 × 10^6 cells in 100 µL.

Question: How should the antibody be stored?

Answer:Store it undiluted at 4 °C, protect it from prolonged light exposure, and do not freeze it.

Application Note

Alexa Fluor® 647 Mouse Anti-Human S100A8/A9 (MRP-8/14), Clone 5.5 is used for intracellular flow-cytometric detection of human S100A8/A9, also known as MRP-8/MRP-14 or calprotectin. The monoclonal antibody uses mouse BALB/c IgG1, κ and was generated against acute monocytic leukemia cells and monocytes. Human reactivity receives quality-control testing, and intracellular staining by flow cytometry is routinely tested. Affinity purification isolates the antibody from tissue-culture supernatant or ascites, and direct conjugation to Alexa Fluor 647 eliminates a separate fluorescent secondary-antibody step. Unreacted dye is removed after conjugation. The reagent arrives prediluted at a working volume of 5 µL per test; a typical test contains 1 × 10^6 cells in a 100 µL experimental sample. Alexa Fluor 647 emission can be collected with the same instrument settings used for allophycocyanin, which supports placement in APC-compatible detector channels during multicolor panel design. The formulation is an aqueous buffered solution containing BSA and no more than 0.09% sodium azide. Store the reagent undiluted at 4 °C, protect it from prolonged light exposure, and do not freeze it. Direct labeling, a defined per-test volume, human QC testing, and established APC-channel collection simplify incorporation into intracellular immunophenotyping panels. The product is for research use only and not for diagnostic or therapeutic procedures.

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