Description
Somatostatin is produced by neuroendocrine neurons in the hypothalamus and δ cells in the islets of Langerhans of the pancreas. It is a regulatory hormone that affects neurotransmission, cell proliferation, and numerous hormones of the endocrine system. Via interaction with high affinity G protein-coupled somatostatin receptors, it inhibits the secretion of somatotropin (also known as growth hormone or GH), thyroid-stimulating hormone (thyrotropin or TSH), and most gastrointestinal and pancreatic hormones, including glucagon and insulin. The expression of somatostatin can be used to monitor the pancreatic differentiation of pluripotent stem cells. The human somatostatin gene, SST, encodes the precursor molecule preprosomatostatin (amino acids 1-116), which is cleaved to form prosomatostatin (amino acids 15-116), which in turn is cleaved to form either of 2 alternative active somatostatin peptides, somatostatin-28 (amino acids 89-116) or somatostatin-14 (amino acids 103-116). The U16-850 monoclonal antibody detects human preprosomatostatin in somatostatin-producing cells.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
