BD, 566613, APC-R700 Mouse Anti-Human LAIR-1 (CD305) DX26

The DX26 monoclonal antibody specifically binds to Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1/ hLAIR1/Leukocyte-associated Ig-like receptor 1) that is encoded by LAIR1, and also known as, CD305. LAIR-1 is a ~32 kDa type I transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory (ITIM) motifs. LAIR-1 is expressed on T cells, B cells, NK cells, monocytes, and dendritic cells. LAIR-1 recruits SHP-1 and SHP-2 tyrosine phosphatases upon activation. Crosslinking of the LAIR-1 expressed on T cells or NK cells can result in strong inhibition of cell-mediated cytotoxicity. Although it is structurally related to human killer cell inhibitory receptors, LAIR-1 does not appear to recognize MHC class I molecules and thus represents a novel MHC class I-independent mechanism of NK cell regulation.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon APC-R700 under optimum conditions, and unconjugated antibody and free BD Horizon APC-R700 were removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.